|
KMID : 0380020080230050413
|
|
Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 5 p.413 ~ p.418
|
|
|
Biological production of 1,3-propanediol using crude glycerol derived from biodiesel process
|
|
|
|
|
Abstract
|
|
|
|
The production of 1,3?propanediol (1,3-PD) was investigated with Klebsiella pneumoniae DSM2026 and K. pneumoniae DSM4799 using crude glycerol obtained from biodiesel industry. Crude glycerol was used without prior purification to investigate effects of impurities in crude glycerol on 1,3-PD production. In the batch cultures, 1,3-PD production with crude glycerol was 1.1~2.5 times higher than that with pure glycerol, indicating that crude glycerol is even a better substrate than pure glycerol for 1,3-PD fermentation. When glucose was added, 1,3-PD production and yield decreased in spite of enhanced cell growth. Futhermore, the addition of glucose was found to increase 2,3-butanediol, a by-product, significantly because of the change in metabolism in the presence of glucose. In semi-batch cultures without glucose addition, 26 g/L 1,3-PD was produced with crude glycerol, which was 2~3 times higher than that with pure glycerol. Based on our results, it was clearly shown that crude glycerol is an effective substrate for biological 1,3-PD production, making it more feasible to produce 1,3-PD at a lower price.
|
|
|
KEYWORD
|
|
|
Biodiesel, Glycerol, 1, 3?propanediol, Klebsiella pneumoniae
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|